Fig. 1

Fig. 1 Fig 1. Wzg, a putative CPS-cell wall ligase, interacts with Wzd and with itself. Interactions between Wze, Wzd, Wzg and WchA were tested using Bacterial Two-Hybrid E. coli system [31]. ?-galactosidase activity of cells expressing putative interaction partners was measured in cell extracts in at least three independent replicates. Black circles indicate median values and brackets show the 25% and 75% percentiles. Positive control (+): E. coli expressing T18 and T25 fragments linked to leucine zipper domains (zip) that can dimerize; Negative control (-): E. coli expressing untagged T18 and T25 fragments. Interactions were detected between T25-Wzg/Wzd-T18 and T25-Wzg/T18-Wzg. ****P???0.0001. As previously described [28] combinations of plasmids expressing Wzd and Wze indicate that these proteins interact.