Fig. 6

LAPTM4B-35 promoting cancer cell dissemination. A, WT, LAPTM4B KO, and LAPTM4B-24? or LAPTM4B-35?stably expressing PC-3 cells were subjected to a wound-healing assay. Images are representative of five independent experiments at indicated time points. Scale bar: 200 ?m. B, Quantification of wound closure of cells treated as in (A). Values represent the average of five experiments, showing percentage of gap closure at indicated times. C, WT, LAPTM4B KO, and LAPTM4B-24? or LAPTM4B-35?stably expressing PC-3 cells were labeled with Celltracker CM-Dil and injected into the perivitelline cavity of WT zebrafish embryos 48 h post fertilization; 36 h post injection, zebrafish were anesthetized and imaged. Cells dissemination from the perivitelline cavity throughout the fish body underwent assessment. Scale bar: 1 mm. D, Metastasis rate of PC-3 cells in zebrafish (fish number with metastases/total fish number). Five independent experiments (PC-3: n = 43; KO: n = 48; LAPTM4B-35: n = 46; LAPTM4B-24: n = 57), p(PC-3, KO) = 0.003, p(KO, LAPTM4B-35) = 0.014