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Fig. 1
LAPTM4B-35 localized on filopodia. (A), LAPTM4B KO cells stably expressing LAPTM4B-35-Flag (designated name in this study: L4B-35) or LAPTM4B-24-Flag (designated name in this study: L4B-24) were labeled with anti-Flag antibody (green), phalloidin-568 (magenta), and DAPI (blue). Scale bar: 10 µm. (B), Quantification of LAPTM4B immunofluorescence intensity in phalloidin-positive filopodia. Quantification of three experiments (LAPTM4B-35: n = 41 cells; LAPTM4B-24: n = 43 cells), mean ± SEM, data normalized to ?L4B-35,? p = 0.015. (C), Number of filopodia in cells stably expressing Flag-tagged LAPTM4B-35 or LAPTM4B-24 on a LAPTM4B KO background. Quantification of three experiments (LAPTM4B-35: n = 41 cells; LAPTM4B-24: n = 43 cells), mean ± SEM, data normalized to ?L4B-35?
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