ZFIN Image: Luo et al., 2022, Fig. 4

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Figure Caption

Fig. 4

Angiogenic switch depends on Vegfa. a The change of FPKM value of RNA-seq data for Vegfa. b RT-qPCR quantification of Vegfa expression in the transplant microtumors during the angiogenic switch (about 12 h and 24 h). c and d Western blot analysis of Vegfa and α-tubulin of B16-Red cells transfected with LentiCRISPRv2-Vegfa, sg1# (Vegfa^{KO 1#}) or sg2# (Vegfa^{KO 1#}) knockout plasmid or pcDNA3.1-VEGFA overexpression plasmid (Vegfa^OE) for 48 h. e and f Imaging of the transplant tumor vascular growth of Vegfa knockout or overexpression B16-Red microtumors. ECs are green. Tumor is red. Scale bar, 50 μm. g-j The branches and length of the first vessel and vessel network. Unpaired t-test, *P < 0.05, ** P < 0.01, *** P < 0.001 (n = 3–5)

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