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Fig. 3
The angiogenic switch does not depend on HIF-1 signaling. a Schematics indicating the microsurgery of dissecting the transplant microtumors and transcriptome sequencing. Avascular, the tumor has not yet induced angiogenesis, which means ‘angiogenic switch’ is off (about 12 h); First vessel, the tumor induces the sprouting of the first vessel, which means ‘angiogenic switch’ is on (about 24 h). b The volcano plot of the gene expression change of the dissected transplant microtumors transcriptome during the angiogenic switch (n = 3). c The GSEA analysis and d the heat map of the HIF-1 signaling pathway during the angiogenic switch (n = 3). e and f The change of FPKM value of RNAseq data for Car9/CA9 and Slc2a1/GLUT1