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Fig. 2
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Figure Caption
Fig. 2. The zygotene cilium is required for bouquet formation.
(A and B) 3D measurements of ciliary lengths and average ciliary length per cyst. n, number of cilia. ND, not detected. Bars are mean ± SD. (C) Distribution of cysts with given ciliary length or absence. (D) Images of cysts from ciliary (AcTub) length categories as in (B) to (D). Percentage of ovaries with indicated categories are plotted. n, number of ovaries. Scale bars, 10 μm. (E) Images of “leptotene-zygotene-like” oocytes of average and large sizes. Scale bars, 5 μm. (F) “Leptotene-zygotene-like” oocyte sizes. n, number of oocytes. Bars are mean ± SD. 9.1 μm represents the largest wt leptotene oocytes. (G) Left panel: images of mid-bouquet-stage oocytes with tight, expanded, and dispersed telomeres (categories shown in top right panel). ND, nondetected. Scale bars, 5 μm. Right bottom panel: the percentage of each category in all genotypes. n, number of oocytes.
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