Figure 7

Figure 7

Blood vasculature extends through cribriform plate with muLEC-like lymphatic cells. (A–E) Sections from Tg(lyve1b:DsRed;fli1a:EGFP) adult brains (n=6 brains examined). (A) I. n dorsal sections the OB is separated from the OE by the cribriform plate (CP). The OB has extensive BV (green) extending into the lamellae of the OE and muLEC-like cells (red) on the surface of the OB (arrow). (A–D) = 100 μm (B). The ON passes through the CP accompanied by extensive BV (green). muLEC-like cells are on the medial surface (red, arrow) of the OB. (C). The muLEC-like cells (red, arrow) line the ventral side of the ON. (D) muLEC-like cells line the basal OE (red, arrows) in the most ventral region of the OO. (E) muLEC-like cells on the BV extending across the CP and many are positive for both lyvel1:DsRed and fli1a:EGFP (arrows). DAPI: white. Scale bar = 50 μm. (F) Diagram depicting olfactory organ with sensory (ss) and non-sensory (ns) epithelia that have extensive BV (green). The lamellae of the OE contain HEV-like LV (red) that do not extend across the cribriform plate (CP). muLEC-like cells (orange) line the BV and extend from the olfactory bulb across the CP to the basal OE. Scale bars: A-D = 100 μm, E = 50 μm.