Figure 6

Figure 6

Blood (BV) and Lymphatic (LV) Vasculature wrap the olfactory organs (OO). (A, B) Whole mount Tg(fli1a:EGFP;lyve1b:DsRed) of the adult OO connected to OB with BV (fli1a:EGFP, green) and LV (lyve1b:DsRed, red). Dorsal (A) and ventral (B) views; DAPI (blue), Scale Bars: (A, B) = 200 μm. (C) BV (red) and LV (green) density is greater in olfactory system (OS = OE and OB) than telencephalon (Tel); (SE, P-value <0.05, unpaired t-test; n = 6 adult brains. One-way ANOVA, Tukey multiple comparison test, P < 0.05). Representative images selected from detailed analysis of least 6 brains. (D) Transmitted light image of fixed whole mount OO. Boxed area corresponds to area where LOE connects with EN. Scale bar = 100 μm, (E). The LV (red) meet the BV (green) at the distal tips of each lamellae (boxed area; asterisks). Scale bar = 100 μm, (F). Cells express both lyve1b:DsRed and fli1a:EGFP (arrows). Scale bar = 25 μm. (A, B), (E, F): Analysis of 9 brains. Two-way ANOVA, Tukey’s multiple comparison test, p < 0.05). (*P, 0.05, ***P, 0.001, ns: non significant).