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Fig. 7
Figure 7. HTR2A agonist promotes melanin synthesis and melanosome transport through the PKA/CREB signaling pathway: (a) Schematic diagram shows the classical signals involved in the initiation of melanogenesis and the process of melanin synthesis and melanin transfer to neighboring keratinocytes; (b) Western blot shows the protein expression of phospho-CREB and total CREB in B16F10 cells after treatment with DOI (30 μM) from 0–14 h; (c) relative melanin content in B16F10 cells treated with DOI (30 μM), H89 (the inhibitor of PKA signaling, 5 μM), and cotreatment as compared with the control (n = 3). The photos of melanin precipitation in the tube are at the top of the diagram (c); (d) Western blot shows the protein expression of MITF and TYR (melanin synthesis), GP100 (melanosome organization), RAB7/17/27 (melanosome transport), RAC1, and CDC42 (melanocytes dendrites) in B16F10 cells treated with DOI (30 μM), H89 (5 μM), and cotreatment. *** (p < 0.001) compared to control group; # (p < 0.05) compared to the group of B16F10 cells treated with DOI.
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