Fig. 5

Fig. 5

RNAseq transcriptome analysis identifies perturbation of ocular development pathways in alx1;alx3 mutants. Larvae derived from alx1^uw2016;alx3²¹¹³/+ parents were lysed individually at 5 dpf for RNA extraction and Illumina high-throughput sequencing. A heat map (A) of significantly dysregulated genes (FDR<0.5) in alx1^uw2016;alx3^uw2113 double mutants relative to alx^uw2016 siblings. A volcano plot (B) of statistical significance against fold-change in alx1^uw2016;alx3^uw2113 mutants relative to alx^uw2016 siblings. Vertical lines indicate −1-fold and +1-fold cut-offs and the horizontal line indicates the FDR=0.05 cut-off. (C,D) The top 20 enriched and top 20 suppressed terms in alx1^uw2016;alx3^uw2113 double mutants relative to alx^uw2016 siblings, identified by GSEA.