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Fig. 2
The expression of dnmt1 is markedly downregulated after knocking down of Dnmt1. A-F, In situ staining of dnmt1 is uniformly downregulated in Dnmt1-MO embryos compared to the controls by WISH at different stages. The black dotted lines outline the otic vesicle (A, C) and neuromast (B, D) at 48 hpf respectively. The black dotted lines in E-F outline the primordium at 32 hpf. G-H, The protein blotting of Dnmt1 is significantly decreased by Dnmt1 morpholino injection both in the band intensity (G) and the quantification analysis (H)
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