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Fig. 7
Figure 7. hGPRC5B-deficient human lymphatic endothelial cells are less proliferative and less viable compared to control cells. (A) Immunofluorescence of human dermal lymphatic endothelial cells treated in vitro with non-targeting control shRNA or shRNA targeting GPRC5B. Left column: red puncta encircled in white indicate Edu-positive cells. Right column: monostaining of EdU-positive nuclei. (B) Quantification of EdU-positive lymphatic endothelial cells. (C) CellTiter-Glo cell viability assay where luminescence indicates viable cells. Significance calculated using one-way ANOVA with correction for multiple comparisons. Scale bar = 50 µm.
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