Fig. 4

Figure 4. Histomorphometric analysis of vascular development reveals attenuated lymphangiogenesis in murine Gprc5b^−/− embryos. Whole-mount immunostaining of dorsal skin from E14.5 Gprc5b^+/+ (top panels (A–D)) and Gprc5b^−/− (bottom panels (A–D)) embryos, stained for the blood vascular marker podocalyxin (A) and the lymphatic marker podoplanin (B–D). Yellow dashed line indicates the dorsal midline (D). Scale bars = 500 µm (E–J). Quantification of total vessel area, total vessel length and relative endpoint counts of lymphatic vessels present at the dorsal back skin midline (E–G) and in the dorsal backskin in non-midline locations (H–J). Significance calculated using unpaired Student’s t-tests.