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Fig. 2 Figure 2. The behavioural responses in the novel tank diving test by zebrafish from holding densities of 1 (n = 15), 4 (n = 35), 8 (n = 65), 12 (n = 104), or 16 (n = 139) fish/L, respectively. Graphs show (a) velocity, (b) total distance moved, (c) latency to the upper zone, (d) latency to the middle zone, (e) frequency of entries to the upper zone, (f) frequency of entries to the middle zone, (g) cumulative duration in the upper zone, and (h) cumulative duration in the middle zone. Data points correspond to individual fish and medians are illustrated as lines. No statistically significant differences were found between the treatments. Each video analysis for the novel tank diving test lasted for 600 s.