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Fig. 6
Knockdown of CYP4V2 increases LDs and free cholesterol accumulation in human RPE-1 cells. (A) Analysis of CYP4V2 and PPARα protein levels in negative control (NC) and siCYP4V2 RPE-1 cells by Western blotting. (B) Quantitative analysis of the Western blot data. At least three independent experiments were performed and quantified. The results are shown as mean ± SD. **P < 0.01. (C) Increased LDs in siCYP4V2 RPE-1 cells as revealed by Nile red staining. Scale bar: 10 µm. (D) Measurement of triglyceride concentrations in NC and siCYP4V2 RPE-1 cells. (E) Filipin staining in NC and siCYP4V2 RPE-1 cells. Scale bar, 10 µm. (F) Free cholesterol concentration in NC and siCYP4V2 RPE-1 cells. The results are shown as mean ± SD. ***P < 0.001; **P < 0.01.
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Full text @ Invest. Ophthalmol. Vis. Sci.