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Fig. 4 Fig. 4. ARPE-19 cells treated with 50 nM OA significantly increased apoptosis visualized by a TUNEL assay. (A) ARPE-19 cells, detected in different conditions, such as untreated, exposed to OA, negative control, and positive control treated with DNase. The samples were stained with TUNEL reagents (red) and co-stained with DAPI (blue) to detect apoptotic cells ; scale 10 µm (B) quantification of apoptotic cells by using 300 cells as a base and presented as a percentage ((number of apoptotic cells/300)× 100). Data was presented as mean ± SEM (n = 6). * ** p < 0.001.
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