Figure 3

Figure 3

Top ten affected canonical pathways following early-life exposure to fluoxetine. The ten pathways with the highest level of significance (i.e. lowest p-value) for each target tissue are shown in larval heads (L), hypothalmus (Hyp), and telencephalon (Tel) in the unstressed (A) and the stressed (B) conditions. The y-axis presents the p-value associated with each pathway on a negative logarithmic scale (-log p-value). The horizontal dash line marks the negative logarithmic value related to the significance level p ≤0.05 [-log10(p)= 1.3]. Each bar shows a canonical pathway identified from the gene cluster enrichment function of IPA. The analysis was performed after converting the gene Ensembl ID of zebrafish (Danio rerio) to human (Homo sapiens) orthologues. Only genes with significant fold change (FC≥1.2; FDR ≤ 0.05) were used for pathway enrichment analysis. (C) The Venn diagram shows the number of all significantly affected pathways in all tissue samples from both the unstressed and the stressed conditions.