Figure 2

Figure 2

The effect of developmental fluoxetine exposure on transcriptome patterns in larval and adult male zebrafish central nervous system in the unstressed and stressed conditions. Hierarchical clustering (Spearman correlation) is shown for all 24 samples (stressed, unstressed, fluoxetine-exposed, and control ethanol-exposed) in a single pooled tissue sample (i.e., larval head, hypothalamus, and telencephalon) and their relation to FLX and stress. (A) FLX-exposed larvae compared to the control larvae. (B) telencephalon from adults exposed to FLX in the early life developmental stage compared to control adults exposed to vehicle compound (ethanol) in the early life developmental stage. (C) hypothalamus from adults exposed to FLX in the early life developmental stage compared to control adults exposed to vehicle compound (ethanol) in the early life developmental stage. The Y-axis consists of all genes with significant fold change (FDR ≤0.05 and FC≥ 1.2) in the treatment groups relative to the control groups. The count per million (CPM) of the reads associated with the green fluorescent protein (d4eGFP) transgene in the SR4G zebrafish line is shown. The different shades of green show different CPM. Red: up-regulation, green: down-regulation. Blue: unstressed condition, Lavender: stressed condition, Light Green: control (ethanol), Pink: Fluoxetine.