ZFIN Image: Karabid et al., 2022, Figure 4

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Figure Caption

Figure 4 Knockout of Tie2 in PitNET cells suppresses tumor growth <italic toggle='yes'>in vivo</italic>

Scheme of the wild‐type Tie2 receptor (left), and the receptor domain left‐over in the targeted clones #18 and #19 (right) and the impact of the mutation on the activation of downstream signaling.

Expression of total Fak and P‐Fak (Y397) in serum‐starved GH3 Ctrl‐KO and Tie2‐KO clones #18 and #19 stimulated with rhANGPT2 for 30 min or left untreated. The numbers represent the ratio phospho/total Fak. Anti‐α‐tubulin antibody was used to check for equal loading. Blot shown is representative of 3 independent experiments.

Scheme of the in vivo study in mouse xenografts of GH3 Ctrl‐KO and Tie2‐KO cells (clone #19).

T2‐weighted images of two xenografted tumors taken at day 0 and 21 (largest diameter) representing the two animal groups. Scale bar: 2 mm, except GH3‐Ctrl KO day 21: 4 mm.

Changes in tumor volumes as determined by MRI were normalized to the day 0 value (=100%) for each animal. All box plots show 25^th to 75^th percentiles (box) and 5^th to 95^th percentiles (whiskers). The line in the box represents the median. **P = 0.003 (one way ANOVA).

Expression of Tie2 (red) in the xenografted tumors (n = 3 each group). Nuclei were counterstained with DAPI (blue). Original magnification: 400×; scale bar: 50 µm.

Source data are available online for this figure.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO Mol. Med.