ZFIN Image: Karabid et al., 2022, Figure 3

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Figure Caption

Figure 3 Tie2 on PitNET cells interacts with Angpt2

Proximity ligation assay (PLA) was performed on FFPE sections of rat PitNETs (n = 5) using antibodies against both Angpt2 and Tie2. Nuclei were counterstained with DAPI (blue). Original magnification: 400×; scale bar: 20 µm.

Quantification of the interactions between Angpt2 and Tie2 in rat PitNET tissues versus negative controls obtained using only 1 antibody (Appendix Fig S7C). Box plots show 25^th to 75^th percentiles (box) and 5^th to 95^th percentiles (whiskers). The line in the box represents the median, whereas the “x” represents the mean.

PLA was performed as in (A) on isolated rat primary NF‐PitNET stimulated with rhANGPT2.

Quantification of Angpt2/Tie2 interactions in primary NF‐PitNET incubated with/without rhANGPT2. The total number of cells counted is reported in parenthesis (i.e., 212 and 345) as well as the number of positive cells (= showing at least one interaction). The graphs show the percentage of cells having the number of interactions reported on the x axis. Box plots show 25^th to 75^th percentiles (box) and 5^th to 95^th percentiles (whiskers). The line in the box represents the median, the “x” represents the mean, and the circle outlier points.

Data information: (C, D) Pictures were taken with the same exposure time. Results shown are representative of the stainings results across all samples (n = 4, n = 3, respectively).

Source data are available online for this figure.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO Mol. Med.