ZFIN Image: Karabid et al., 2022, Figure 2

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Figure Caption

Figure 2 Tumor cells‐secreted Angpt2 induces angiogenesis <italic toggle='yes'>in vivo</italic>

Zebrafish larvae were implanted with red fluorescence‐stained GH3 cells infected with unspecific shRNA (shCtrl) or with shAngpt2 (#2). In the bottom panels, the red channel was omitted to highlight the tumor‐induced microvascular networks. Digital magnifications of graft regions (white boxes) are shown in the bottom left panels. Scale bar: 100 µm.

Quantification of tumor‐induced vessel sprouting in zebrafish embryos engrafted with GH3 ‐shCtrl versus shC2 cells (n = 20 each). Data were normalized against the mean of the control (shCtrl) group arbitrarily set to 1. A.U. arbitrary Units; *P = 0.0319 (t‐test).

Example of flow cytometry data of primary rat pituitary cells from a tumor‐bearing MENX rat gated for cell surface Tie2 and CD31 expression (cells were pre‐gated for Tie2).

Percentage of Tie2⁺CD31⁻ (PitNET cells) and Tie2⁺CD31⁺ (ECs) in the pituitary glands of 7 age‐matched (8 months) MENX rats. Data are expressed as the mean ± SEM. ****P < 0.0001 (t‐test).

A representative graph displaying the Tie2 fluorescence intensity and counts of Tie2⁺CD31⁻ and Tie2⁺CD31⁻ cell populations the pituitary of one rat.

Fluorescence intensities of the Tie2⁺CD31⁻ and Tie2⁺CD31⁻ cell populations across all 7 pituitary samples. Data are expressed as the mean ± SEM. n.s, not significant (Mann–Whitney test).

Source data are available online for this figure.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO Mol. Med.