ZFIN Image: Karabid et al., 2022, Figure EV1

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Figure Caption

Figure EV1 Angpt2 is expressed in rat primary NF‐PitNETs together with Tie2 and Angpt1

qRT–PCR for Angpt1 and Angpt2 was performed on pituitary samples from tumor‐bearing MENX mutant rats (n = 10, 12, respectively) and wild‐type control rats (n = 5, 8). Data are expressed as mean ± SEM. *P = 0.049; **P = 0.008 by t‐test.

IHC was performed on pituitary tissues from wild‐type (n = 5) and MENX mutant rats (n = 10) using antibodies against Angpt1, Angpt2. Representative stainings are shown. Original magnification: 400×; scale bar: 20 µm. N, normal area; T, tumor area.

Expression of Angpt2, Tie2, and CD‐31 in rat NF‐PitNETs and associated ECs (used as positive control). Consecutive tissue sections of rat NF‐PitNETs (n = 4) were stained with the indicated antibodies. One representative tumor is shown. The dashed shape indicates the vessel present in the consecutive slides. Original magnification: 200×; scale bar: 20 µm.

Acknowledgments

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