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Figure 3—figure supplement 2. Final lengths of airinemes.

For the model parameter lmax, which is the length at which an airineme stops searching if it has not yet found a target, we wondered whether a single number would be appropriate or whether we needed to use a distribution to capture the variability of airineme lengths. We measure the experimental quantity lfinal, which is the length of airinemes when they stop growing, whether or not they have made contact with a target cell, shown in red in (A, B) (N=56 airinemes). We run simulations in which target cells are randomly placed according to 2D Poisson statistics with mean distance dtarg=50μm and measured their length distribution when they stop growing lfinal, shown in blue in (A, C) (again, whether or not they have made contact with a target). We find that the distributions for experimental data and simulated data agree qualitatively when lmax=250μm. Note that the variability in both experimental and simulated distributions is of similar magnitude, suggesting that the randomness in observed airineme length arises from the randomness in target cell placement.

Acknowledgments
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