Fig. 1

Fig. 1 (A) Represents WST assay to check for antiproliferative and cytotoxic nature of monacolin X on human endothelial cells-HUVECs and EA.hy926. All the cells were treated with monacolin X at various concentrations (0–300 μM) for 24 h. (B) Cell membrane integrity by release of lactate dehydrogenase (LDH) activity by LDH assay. HUVECs and EA.hy926 cells were treated with monacolin X at various concentrations (0–300 μM) for 24 h. LDH released into the medium was measured along with blank, untreated cells (0 μM), had low LDH release in media and whereas treated cell had dose dependent release of LDH. (C) The morphological analysis of HUVECs treated with monacolin X at IC₅₀ concentration (62.77 μM) for 24 h. Control, monacolin X treated and SU5416 treatment respectively. Morphological changes of control and monacolin X treated HUVECs cells evaluated with PI staining by fluorescence microscopy. The percentage of necrotic nuclei after 24 h treatment with monacolin X treated increased enormously, as revealed by nuclear condensation and fragmentation. Apoptotic and nuclear morphological changes in HUVECs cells treated with monacolin X evaluated with AO/EB dual staining.