Phosphatidylcholine (PC) and phosphatidylethanolamine (PE) species in sperm from lpgat1+/− zebrafish. PC (A) and PE (B) species composition of zebrafish sperm was analyzed by LC–MS/MS. Semen was collected from an anesthetized zebrafish by abdominal compression. Sperm pellets were prepared from semen collected from one zebrafish and lipids were recovered. The numbers of sperm samples were n = 4 for lpgat1+/+ and n = 4 for lpgat1+/−. Error bars are S.D. Statistically significant differences are marked with asterisks. *p < 0.05; **p < 0.01; ***p < 0.001. Unpaired, two-tailed t test was used. For molecular species with low abundance, the relative abundance of each molecular species is shown in Supplementary Fig. S12.
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