IMAGE

Fig. 5.

ID

ZDB-IMAGE-220509-10

Genes

thbs4b

Antibodies

Publication

Blackwell et al., 2022 - Hnrnpul1 controls transcription, splicing, and modulates skeletal and limb development in vivo

All Figures

Figures for Blackwell et al., 2022

Image

Figure Caption

Fig. 5.

Loss of hnrnpul1l and hnrnpul1 leads to caudal scoliosis in adult Zebrafish. a, b) Muscle (MyHC, blue) and tendon (Tbsp4b, magenta) staining of 72 hpf wild type (a, a’’) and hnrnpul1/1l mutant (b, b’’) embryos, arrowheads highlight less organized muscle fibers in hnrnpul1/1l mutants. c, c’) Alcian blue cartilage and alarizin red bone staining of 16 dpf wild type and hnrnpul1/1l. d, d’) Alizarin red bone staining of 16-week-old adult wild type (d) and hnrnpul1/1l mutant (d’) fish. e, e’’’) Alizarin red bone staining of 16-week-old adult wild type and hnrnpul1/1l mutants graded by scoliosis in the caudal (tail) region (arrowheads) as none (e), mild (e’), moderate (e’’), and severe (e’’’). Schematics show spine curvature from the images above. f) Quantification of the proportion of fish with none, mild, moderate, or severe scoliosis. Wild type n = 39, hnrnpul1/1l mutant n = 45, from 3 trials. **P ≤ 0.01, ****P ≤ 0.0001 determined by Fisher’s exact test. Scale bar = 1000 µm (e, e’’’).

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ G3 (Bethesda)