Figure 4

Figure 4

Zebrafish IRF family members show three patterns of constitutively subcellular localization. (A–D) Confocal microscopy illuminates the subcellular localization of zebrafish IRF proteins. HEK293T cells seeded on microscope slide cover glasses overnight in six-well plates were transiently transfected with pEGFP-N3 as control (A), or with each of GFP-fused IRF1/2/9/11 constructs (B), or with each of GFP-fused IRF3/5/7 constructs (C), or with each of GFP-fused IRF4b/6/8/10 (2μg each). At 24 h post transfection, cells were fixed and examined using a confocal microscopy. DAPI staining showed the nuclei. The last column showed magnification view of the area highlighted in the box. (E) The intensities of nucleus/cytoplasm GFP were quantitated by the ImageJ processing program followed by normalization to that of pEGFP-N3, which was set to 1:1.