Figure 3

Figure 3

Zebrafish IRF proteins bind to zebrafish promoter DNA. (A) Schematic diagram of zebrafish IFNφ1 and IFNφ3 promoters, showing the position and sequence of potential ISRE/IRF-E motifs. (B) The binding of zebrafish IRF proteins to zebrafish IFN promoter DNA. The biotin-labeled DrIFNφ1 (-586 to +38) or DrIFNφ3 (-1447 to -910) promoter DNA (20 ng each) was incubated with the excessive amounts of zebrafish IRF proteins, or with GFP as control. Zebrafish IRF and GFP proteins were derived from the lysates of HEK293T cells that had been transfected for 30h with plasmids expressing zebrafish IRF protein fused to GFP or pEGFP-N3, respectively. One-tenth of cell lysates were taken as input. The bead-bound DNA-protein complex was detected by the Ab specific to the GFP tag using Western blotting.