ZFIN Image: Ouyang et al., 2022, FIGURE 1

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Figure Caption

FIGURE 1

Generation of the vmhc^KI–EGFP zebrafish KI line. (A) Schematic diagram of the KI strategy based on the HR pathway triggered by the CRISPR/Cas9 system. The TAA stop codon (in red) is included in the guide RNA target sequences. Protospacer adjacent motif (PAM) sequences are marked in green. The donor vector used for HR contains the left homologous arm, P2A-EGFP, and right homologous arm. Restriction enzymes sites used for left and right homologous arms cloning are marked in blue. (B) Correct integration of P2A-EGFP into the C-terminal of the endogenous vmhc locus was confirmed by genotyping PCR and Sanger sequencing in the F1 stable KI embryos.

Acknowledgments

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