FIGURE 2

FIGURE 2

Fraction 2 of CH₂Cl₂ extract suppresses adipogenesis in zebrafish VAT. (a) Flow chart showing elution fractions obtained using silica gel chromatography. (b and c) Zebrafish obesogenic test. Fraction 2 (10 μg/ml) reduced the amount of VAT compared to control treatment (0.1% DMSO). Data are shown as the means ± standard deviation. n = 5, *p < .05 versus control, as calculated by using one‐way ANOVA. Reproducibility test results are provided in Figure S3. (d and e) qPCR analysis of early adipocyte differentiation markers CCAAT/enhancer‐binding protein beta (cebpb) and CCAAT/enhancer‐binding protein delta (cebpd; d) and late differentiation markers peroxisome proliferator‐activated receptor gamma (pparg) and CCAAT/enhancer‐binding protein alpha (cebpa; e). Data are shown as the means ± standard deviation. n = 5 or 6, **p < .01 versus control, as calculated by using the Student's t‐test