Figure 4

Figure 4

miR-22a regulates primary motor neuron and ISV pathfinding. (a) Confocal imaging analysis of fli1a:miR-22a-sponge-injected Tg(mnx1:EGFP::kdrl:ras-mCherry) embryos at 72 hpf. (b) Percentage of embryos with impaired ISV and PMN pathfinding phenotype in fli1a:miR-22a-sponge-injected embryos at 48 hpf and 72 hpf, respectively (n = 108; n = 120). (c) Confocal imaging analysis of huc:miR-22a-sponge-injected Tg(mnx1:EGFP::kdrl:ras-mCherry) embryos at 72 hpf. (d) Percentage of embryos with indicated phenotypes in huc:miR-22a-sponge-injected embryos at 48 hpf and 72 hpf, respectively (n = 112; n = 105). (d) Quantitative PCR analysis of miR-22a in control neuron cells and neuron cells sorted from embryo injected with ECs expressing miR-22a-sponge at 24 hpf (n = 3), 48 hpf (n = 3), and 72 hpf (n = 3). t-test; **p < 0.001.