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Sheng et al., 2022 - MicroRNA-22 coordinates vascular and motor neuronal pathfinding via sema4 during zebrafish development

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Figure 3

Deficiency of mir-22a caused aberrant axonal projection of PMNs. (a) Confocal imaging analysis of primary motor neurons in the control embryos, miR-22a morphants and Tg(mnx1:GFP::ubi:miR-22a-sponge) embryos at 48 and 72 hpf. Arrowheads indicate the CaPs across different segments. (b,c) Schematic diagram for primary motor neurons in the control embryos and miR-22a morphants. (d) Statistical analysis of the ratio of CaPs across different segments in the control (n = 8), miR-22a morphants (n = 8) and Tg(mnx1:GFP::ubi:miR-22a-sponge) embryos (n = 8) at 48 and 72 hpf; One-way ANOVA, ****p < 0.0001. (e) Statistical analysis of the ratio of aberrant axonal projection of CaPs in the control (n = 8), miR-22a morphants (n = 8) and Tg(mnx1:GFP::ubi:miR-22a-sponge) embryos (n = 8) at 48 and 72 hpf; one-way ANOVA, ****p < 0.0001.

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