Figure 4

Figure 4

MITF promoter activity is reduced under expression of PPP6C(WT) and PPP6C(R264C) on multiple oncogenic backgrounds. (A, C, E) Representative confocal imaging of fluorescent pigment less melanocytes of tgBRAF(V600E); nacre;p53(M214K);albino, roy;nacre, or roy;nacre;p53(M214K) fish injected with a GFP reporter under control of the mitfa promoter. (B, D, F) Representative quantification of fluorescence for each genotype. Fish were imaged under a confocal microscope and total fluorescence was quantified for 10 melanocytes on N = 5 fish of each genotype. All data panels in the figure are representative of at least three experiments (Student’s two-tailed t-test). p-values are indicated as following: *p < 0.05, ***p ≤ 0.001, ****p ≤ 0.0001.