FIGURE 4

FIGURE 4

Effects of aconitine on TH, MAO, DAT and VMAT2 expressions in zebrafish larvaes and SH-SY5Y cells. (A) The mRNA expressions of th, mao, dat, vmat2 with aconitine exposure in zebrafish larvaes, n = 6. (B) TH and VMAT2 positive cells were investigated using immunostaining and Tg (vmat2:GFP) transgenic zebrafish larvaes, respectively, n = 5. (C) Effect of different exposure times of 1 μM aconitine on the protein expression of TH, n = 4. (D) The protein levels of TH, MAO-B, DAT and VMAT2 with aconitine exposure in zebrafish larvaes, n = 4. (E) The mRNA expressions of th, mao-b, dat, vmat2 with aconitine exposure in SH-SY5Y cells, n = 3. (F) The protein levels of TH, MAO-B, DAT, and VMAT2 with aconitine exposure in SH-SY5Y cells, n = 7. All photographs in which the green fluorescence represented the immune-reactive cells were taken under a confocal microscope (100×). The values are expressed as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 vs. control groups.