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Figure 4
Figure 4. Detection of ear morphological defects in zebrafish models. (A) Dorsal view of zebrafish at 72 hpf. (B) Lateral view of the posterior otolith at 72 hpf zebrafish (black arrow). (C) Closeup view of zebrafish ear morphology (black dashed circle). (D) The perimeter length of zebrafish ear, utricle, and saccule area in μm2. The total number of experiments was 3, and the number of embryos analyzed was 31, 18, 19, and 36 for control, ZF_myo6a_MO, MYO6WT, and MYO6p.E60Q groups, respectively. One-way ANOVA using GraphPad Prism software (version 8.0) and Tukey’s multiple comparisons test with p-values of * p < 0.05, ** p < 0.01, *** p < 0.001.
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