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Figure 7—figure supplement 2.

ID: ZDB-IMAGE-220326-23
Publication: Vasileva et al., 2022 - Dysregulated heparan sulfate proteoglycan metabolism promotes Ewing sarcoma tumor growth
All Figures: Figures for Vasileva et al., 2022

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Figure Caption

Figure 7—figure supplement 2. Changes in the fin shape driven by <italic toggle='yes'>EWSR1-FLI1</italic> expression as a readout for drug screening.

(A) Changes in the fin shape driven by EWSR1-FLI1 expression. Embryos injected with ubi:RSG-EWSR1-FLI1 plus GFP mRNA were used as control. (B) Scheme of surfen treatment. Wild-type embryos were used for the integration of EWSR1-FLI1 into the zebrafish genome. Uninjected fish were used as control. eGFP-positive embryos were treated with surfen at 0.2 μM or 0.2% DMSO vehicle control. Zebrafish were imaged after 48 hr of treatment. Coefficient of curvature was calculated as following Ccurv = (L1 − L0)/L0 × 100, where L1 is the length of fin edge between points A and B and L0 is the length of the straight line between those two points. (C) Fins representing different coefficients of curvature Ccurv caused by EWSR1-FLI1 expression. (D) Treatment of fish expressing EWSR1-FLI1 with surfen leads to the rescue of fin curvature phenotype. Error bars represent SD, biological replicates N = 4, **p < 0.01, ***p < 0.001, ****p < 0.0001 based on one-way analysis of variance (ANOVA) test.

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