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Figure 1—figure supplement 2.

ID: ZDB-IMAGE-220326-12
Publication: Vasileva et al., 2022 - Dysregulated heparan sulfate proteoglycan metabolism promotes Ewing sarcoma tumor growth
All Figures: Figures for Vasileva et al., 2022

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Figure Caption

Figure 1—figure supplement 2. The Tol2 transposon-based system was used to integrate <italic toggle='yes'>b-actin:RSG-EWSR1-FLI1</italic> into the wild-type zebrafish genome.

Coinjection of Cre mRNA or GFP mRNA was used to generate EWSR1-FLI1-expressing or control zebrafish, respectively. (A) TUNEL assay (red) made on zebrafish expressing eGFP (green) or eGFP2A-EWSR1-FLI1 (green) at 36 hpf. (B) Relative mRNA expression of EWSR1-FLI1 in b-actin:RSG-EWSR1-FLI1 zebrafish injected with Cre RNA or GFP RNA. (C) Expression of EWSR1-FLI1 on the protein level was confirmed by immunoblotting. (D) Representative images of zebrafish expressing EWSR1-FLI1 driven by b-actin promoter at 1 mpf. (E) H&E staining of cell masses formed in the head of zebrafish expressing EWSR1-FLI1 under b-actin promoter (right) and control (left). Error bars represent standard error of the mean (SEM), N = 3, ****p < 0.0001, two-tailed Student’s t-test.

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