Figure 1
- ID
- ZDB-IMAGE-220326-10
- Publication
- Vasileva et al., 2022 - Dysregulated heparan sulfate proteoglycan metabolism promotes Ewing sarcoma tumor growth
- All Figures
- Figures for Vasileva et al., 2022
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Figure 1
(A) Overview of experimental method. The Tol2 transposon system was used to integrate human EWSR1-FLI1 into the wild-type zebrafish genome by microinjection into single-cell-stage embryos. (enhanced Green Fluorescent Protein) eGFP-positive fish were monitored up to 4 months. (B) Constructs for Cre-inducible expression of EWSR1-FLI1. (C) Incidence of eGFP+ tumors detected in Ubi:RSG-EWSR1-FLI1; Cre RNA (n = 77) injected zebrafish versus Ubi:RSG-EWSR1-FLI1; eGFP RNA (n = 142) and uninjected controls (N = 150) in a wild-type genetic background (p < 0.001 by log-rank test). (D) Representative images of eGFP-positive tumors in zebrafish (top panel) and H&E staining of tumor sections (bottom panel): (I) SRBCT with diffuse skeletal muscle infiltration and (II) visceral SRBCT arising from fin dorsal radial bone. Scale bars, 100 μm. (E) Percent tumor incidence at different anatomic sites.
Cre-inducible expression of EWSR1-FLI1 drives small round blue cell tumor (SRBCT) development in zebrafish.