ZFIN Image: Kiledjian et al., 2022, Figure 7.

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Figure Caption

Figure 7.

Mutant sbp2 ^−/− larvae and general selenoprotein production are sensitive to peroxide treatment.

(A) Wild-type (blue) or mutant (orange) larvae (n = 110) were raised in embryo medium with or without 2 mM hydrogen peroxide for 5 d (water was changed daily). Survival was analyzed by the Kaplan-Meier method and P-values were determined by the log-rank test. (B) 3–5 days post fertilization wild-type (wt) or secisbp2l^−/− (Δ) larvae were incubated with 375 nM ⁷⁵Se with or without 500 μM hydrogen peroxide for 24 h. Radiolabeled proteins were analyzed by SDS–PAGE followed by phosphorimaging (top panels) and total protein is shown as fluorescence imaging of TCE-stained gels (bottom panel). (C) Quantitative analysis of selenoprotein production in the presence or absence of peroxide. (B) ⁷⁵Se incorporation was determined by quantitation of the phosphorimages shown in (B). Data were normalized to wild-type untreated samples and shown as the average of three experiments plus/minus SD.

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