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Fig. 7 Cardiac alterations of natterin-like depleted (KO) zebrafish larvae. The KO or the wild-type (WT) larvae had the heart area and heartbeat rate measured at 24, 48, 72, 96, 120, and 144 h post-fertilization (hpf; 20 larvae per time). Pericardial measurement was evaluated using the ImageJ software from images obtained in a Leica M205C stereomicroscope. The data represent the average of each measurement at the designated time (A; 20 larvae per group). The beat rate was counted in 15 s videos acquired on the Leica M205C stereomicroscope at 50x magnification (LAS V4.11 software). Dot plot data show heart rate individually and the bars represent the mean of each group plus the standard deviation (B). The asterisks (*) represent a significant difference with the WT control (p < 0.05)