Figure 2.

Figure 2.

Increased β-cell mass in Rest^pKO mice. (A) Representative images of 10 × 10 frame reconstructions used for β-cell morphometry of insulin (red) and DAPI (green) stainings in pancreas from 12-wk-old control and Rest^pKO mice. Scale bar, 2 mm. (B) Morphometry of β-cell mass estimated from insulin surface area/total DAPI surface area (percentage). Rest^pKO mice have an approximately twofold increase in β-cell mass. n = 12 sections from five to six mice in each group. (C) Islet size in control and Rest^pKO adult pancreas. (D) Representative immunofluorescence for glucagon (red), insulin (green), and DAPI (blue) in whole-pancreas from 12-wk-old control and Rest^pKO pancreas. Scale bar, 200 µm. Error bars indicate SEM. (**) P ≤ 0.01.