Figure 4

Figure 4

(A) The setup implementation in the Flow LSFM modality with double simultaneous detection scheme. (B) The imaging chamber embedding two sealed water detection objective (DO) and a FEP tube crossing it at 45°. SF sample flow. (C) Exemplification of the sample mounting and scanning system. The sample flows within the FEP tube through a vertical light-sheet. (D) Depth-colored maximum intensity projection of a fixed tissue spheroid expressing a histone H2B fluorescent nuclear reporter protein (mCherry), obtained through single (left) and double (right) side detection scheme. The fusion of two views (on the right) reveals nuclei that would be otherwise missed through single side detection (see white arrows). Scale bars 100 µm. (E) Series of images during the flow of a Fli-GFP zebrafish embryo through the system. The front view (FV) and the back view (BV) are simultaneously registered on the same camera chip. Scale bar 100 µm. (F) 3D reconstruction view of the same zebrafish showed in (E). Colors represent signal intensity (Fire LUT in Fiji). (A) created with FreeCAD 0.16 (www.freecadweb.org) and (C) created with Autodesk Inventor Professional 2015 (www.autodesk.com).