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Figure 2 (A) The objectives? configuration enabling multiple illumination/detection schemes. (B) Through the first mounting option the sample is embedded within a cylinder perpendicular to the optical table. The black arrow indicates the scanning direction. (C) The second approach uses the flow of the samples to scan them through the light sheet. The red arrow indicates the flow direction. (D) Through the third approach, the sample flow (red arrow) is used to position the sample in the objective?s FoV of the DO. The scanning of the specimen is obtained instead in the vertical direction (black arrow), either mechanically or optically. IO illumination objective, DO detection objective, LS light sheet, FC fluidic circuit. Figure created with Autodesk Inventor Professional 2015 (www.autodesk.com).