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FIGURE 11 Double mutant of egfra and inha (egfra−/−; inha−/−) induced ovarian fibrosis and hydrovarium. The egfra+/− and inha−/- females were used as the control. Follicles were undergoing PG-PV transition at 45 dpf in both control and double mutant. These follicles continued to grow in the control ovary at 60 dpf with significant yolk accumulation in leading oocytes; however, the growth stopped completely at PG and early PV stages in the double mutant ovary (egfra−/−; inha−/−), which also showed fibrosis and accumulation of somatic stromal cells between follicles. The ovarian lamellae started to exhibit hydrovarium in the dorsal parts at 75 dpf in the double mutant, and the amount of fluid (asterisk) and fibrosis (arrowhead) continued to increase at 90 dpf. PG, primary growth; PV, previtellogenic; EV, early vitellogenic; MV, mid-vitellogenic; LV, late vitellogenic; FG, full-grown.