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FIGURE 10 Development of secondary sexual characteristics during sex reversal and fertility test for sex-reversed males. (A) Protruding genital papilla (upper left, arrow) and a light anal fin (lower left) were observed in egfra−/− females at 6 mpf. The genital papilla disappeared and the anal fin turned golden in color in the same fish at 13 mpf. (B) The sex-reversed males (egfra−/−) showed normal testis and spermatogenesis at 13 mpf. (C) The sex-reversed males were fertile and could spawn with WT females to produce normal embryos. (D) All the offspring of sex-reversed males and WT females were heterozygous egfra+/− as genotyped by HMA.