ZFIN Image: Dadras et al., 2021, Fig. 7

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Figure Caption

Fig. 7

Inhibiting aPKC or inducing mitochondrial ROS disrupts gliomasphere formation.

a Intracellular ROS content measured by DCFH-DA fluorescence after treatment of U3031MG cells with 1 µM aPKCi and expressed as a percent of control after 3 days. b ELDA expressing median values from U3031MG treated with 1 µM aPKCi for 10 days (Control, black curves; aPKCi treated, red curves). Note the large degree of shift of the median curves to the right upon aPKCi treatment. The table shows the stem cell frequency (1 stem cell/x cells); n = 3 with six replicates. c Intracellular ROS content measured by DCFH-DA fluorescence after treatment of U3031MG cells with 100 µM MitoPQ for 3 days and expressed as a percent of control. d ELDA expressing median values from U3031MG treated with 100 µM MitoPQ for 10 days (Control, black curves; MitoPQ treated, red curves). Note the large degree of shift of the median curves to the right upon MitoPQ treatment. The table shows the stem cell frequency (1 stem cell/x cells); n = 3 with six replicates. e Par3 localization in proximity to mitochondrial networks revealed by in situ PLA. MitoTracker Deep Red staining of mitochondria is represented in green, Par3 molecules are represented as single red dots and nuclei are visible in blue. Insets (dotted rectangles) magnify a single cell for a better visual effect. Arrows indicate co-localization. Magnification bars, 10 µm.

Acknowledgments

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