ZFIN Image: Dadras et al., 2021, Fig. 5

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Fig. 5

Par3 regulates ROS and mitochondrial function in GBM cells.

U3028MG, U3031MG, and U3034MG cultures were transfected with control (siC, black bars) and Par3 (green bars) siRNAs. a Intracellular ROS content measured by DCFH-DA fluorescence is expressed as percent of the control siRNA (siC) on each day. For U3028MG, n = 2 in four duplicates for day 1, n = 2 in two duplicates for day 3; for U3031MG, n = 2 in four duplicates for days 1 and 3; for U3034MG, n = 3 in four duplicates for day 1, n = 2 in four duplicates for day 3. b Mitochondrial ROS measured by MitoSOX-Red is expressed as percent of the control siRNA (siC). For U3031MG and U3034MG, n = 5 in triplicate; for U3028MG, n = 3 in triplicate. c Mitochondrial transmembrane potential analysis using MitoTracker CMXROS at day 3. For U3034MG: n = 4; for U3028MG and U3031MG, n = 2. d ATP levels in U3028MG and U3034MG cells expressed as percent of the control siRNA (siC). For U3028MG, n = 2; for U3034MG, n = 4. All results are expressed as mean ± SEM of at least two repeats; statistical comparison (t-test) indicates significant differences, *p < 0.05, **p < 0.01, **p < 0.001.

Acknowledgments

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