ZFIN Image: Tatarakis et al., 2021, Figure 6.

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Figure Caption

Figure 6.

Bulk RNA-seq of a transgenic Wnt reporter combined with scRNA-seq analysis reveals temporal kinetics of Wnt signaling

(A) Diagram showing experimental design. Lateral views of the head of a sox10:EGFP;7xTCF:nls-mCherry transgenic show migrating NC cells with either high (high Wnt) or low (low Wnt) mCherry levels were isolated via FACS at 24 hpf and then combined for bulk RNA sequencing. Differentially expressed (DE) genes were then analyzed using our scRNA-seq timeline to uncover temporal patterns.

(B) Heatmap showing DE genes between high-Wnt and low-Wnt cells.

(C) Wnt-upregulated genes were clustered based on their temporal profiles in the scRNA-seq timeline using DPGP (Dirichlet Process Gaussian Process). The timeline (x axis) was split into pigment (right panels) and non-pigment (left panels) branches to separate out Wnt-regulated genes that specifically drive pigment specification. For each branch, two major clusters of genes have either early (lower panels) or late (upper panels) expression signatures. Two genes with these profiles from each temporal cluster are indicated as examples within the plots.

Acknowledgments

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