ZFIN Image: Wu et al., 2021, G

Image

Figure Caption

G AG activates ERK to induce MMPs

A

Immunoblots of cell lysates were performed to analyze p‐ERK1/2, p‐JNK, p‐P38, and p‐P65 by THP‐1 cells stimulated with AG (1 μg/ml) for indicated times, and GADPH as a loading control.

B

Immunoblots of cell lysates were performed to analyze p‐ERK1/2, p‐JNK, p‐P38, and p‐P65 by mouse peritoneal macrophages stimulated with AG (1 μg/ml) for indicated times left untreated or pretreated with AG aptamer (1 μg/ml), and GADPH as a loading control.

C–E

qPCR analysis of Mmps including Mmp9 (C), Mmp10 (D), and Mmp12 (E) from THP‐1 cells stimulated with AG (1 μg/ml) for 24 h in the absence or presence of different inhibitors targeting NF‐κB (PDTC), ERK (PD98059), JNK (SP600125), and p38 (SB203580) at the concentration of 10 μM.

F

Immunoblots of cell supernatants to analyze secreted MMP9, MMP10, MMP12, and MMP13 by mouse peritoneal macrophages stimulated with AG (1 μg/ml) for indicated times in the absence or presence of inhibitor targeting ERK (PD98059) at the concentration of 10 μM; GADPH of cell lysates served as the loading control.

G

Immunoblots of cell supernatants to analyze secreted MMP9, MMP10, MMP12, and MMP13 by mouse peritoneal macrophages infected with H37Rv for indicated times (MOI = 5) in the absence or presence of inhibitor targeting ERK (PD98059) at the concentration of 10 μM; GADPH of cell lysates served as the loading control.

Data information: Data in (A and B) are representative of n = 3 independent experiments. Data in (C–E) are means ± SD averaged from n = 3 independent experiments performed with technical triplicates, and each symbol represents the mean of technical triplicates. Two‐way ANOVA followed by Dunnett's post hoc test (C–E) were used for statistical analysis. ns, not significant; *P < 0.05; ****P < 0.0001.

Source data are available online for this figure.

Acknowledgments

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