ZFIN Image: Wu et al., 2021, D

Image

Figure Caption

D–G AG is a virulence factor of mycobacteria

A, B

C57BL/6 mice were left untreated (NT) or were intraperitoneally treated with indicated amounts of AG for 3 days. Lung sections stained with hematoxylin and eosin(H&E) (A) and quantification of lung lesion burden from H&E‐stained sections (B).

C

Sequence and secondary structure of identified aptamers against AG as predicted with DNAMAN version 6.0.

D–G

H&E staining of lung sections from mice 4 weeks after intranasal infection with M. bovis BCG (D and E) or Mtb H37Rv (F and G) in the absence or presence of intranasally administrated AG aptamers (1 μg) once at a 1‐week interval. Quantification of lung inflamed regions shown in (E and G).

Data information: Data in (B, E, G) are means ± SD of indicated numbers of mice from 1 of n = 3 independent experiments with similar results and each symbol represents 1 mouse. Data in (A, D, F) are representative of n = 3 independent experiments. One‐way ANOVA followed by Dunnett's post hoc test (B and E) and Student’s t‐test (G) were used for statistical analysis, respectively. ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Scale bar, 200 μm.

Acknowledgments

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